Faculty

Although they are only present at part-per-million to part-per-billion levels, trace metals are absolutely essential for life. Our research is focused on understanding the many roles that trace metals (especially Mn, Fe, Co, Ni, Cu and Zn) play in biology.

A portion of this work focuses on the biochemistry of metalloenzymes (proteins with metals at their active sites). Metalloenzymes catalyze reactions with a speed and selectivity that is unrivaled by conventional catalysts. We want to understand how metalloenzymes work. Our approach is to correlate metal-site structure with enzymatic function, with particular interest in Zn containing proteins. Zn has recently been shown to play a critical role in catalyzing the transfer of alkyl groups to thiol acceptors, forming thioethers. The enzymes, which are involved in a number of critical reactions, ranging from homocysteine homeostasis to protein farnesylation to alkene metabolism, generally contain thiolate-rich Zn sites. We want to understand the mechanism of Zn-promoted alkyl transfer. How does Zn(II) promote nucleophilic attack of a thiol on an alkyl donor?

In addition to their enzymatic roles, essential trace elements play numerous other roles in biology and fluctuations in metal concentration are associated with numerous disease states. We have developed techniques that allow us to follow the distribution and chemical speciation of first row transition metals in intact tissue. Using an X-ray nanoprobe, we have been able to map sub-cellular distributions of metal ions with spatial resolutions as good as 100nm. Using x-ray absorption spectroscopy, we are able to determine the chemical speciation of these metal ions in intact biological tissues. The ultimate goal of this work is to develop the new field of inorganic physiology - the study of the transport, storage and distribution of metal ions in biology.

We make extensive use of synchrotron radiation, using the unique resources available at synchrotron laboratories in the U.S. (Brookhaven, Argonne, Stanford and Berkeley) and abroad (Japan, France). A key technique is X-ray absorption spectroscopy. This is one of the only ways to obtain detailed structural information for non-crystalline systems. In addition to X-ray methods, we make use of a wide range of other spectroscopies, including EPR, IR and paramagnetically-shifted NMR.

Awards

Farrel Lytle Award, 2003
Akron Section Award (American Chemical Society), 2000
Fogarty Senior International Fellowship, 1998 -1999
University of Michigan, Faculty Recognition Award, 1994
Alfred P. Sloan Research Fellow, 1991-1993
US Young Investigator for the IUPAC assembly, 1988
Camille and Henry Dreyfus Foundation Distinguished New Faculty Award Recipient, 1985
National Science Foundation pre-doctoral fellowship, 1979-1982
Elected Fellow of the AAAS

Representative Publications

1. Landmark, K.J., DiMaggio, S., Ward, J., Bogt, S., Hong, S., Kotlyar, A., Penner-Hahn, J.E., Baker, J.R., Banaszak Holl, M.M., Orr, B.G., "Synthesis, Characterization, and In Vitro Testing of Superparamagnetic Iron Oxide nanoparticles Targeted Using Folic Acid-Conjugated Dendrimers", ACS Nano, 2008, in press.

2. Xue, Y., Davis, A.V., Balakrishnan, G., Stasser, J.P., Staehlin, B.M., Focia, P., Spiro, T.G., Penner-Hahn, J.E., O'Halloran, T.V., "Cu(I) recognition via cation-pi and methionine interactions in CusF", Nature Chem. Biol., 2008, 4, 107.

3. Matzapetakis, M., Ghosh, D., Weng, T.C., Penner-Hahn, J.E., Pecoraro, V.L., "Peptidic Models for the Binding of Pb(II), Bi(III) and Cd(II) to Mononuclear Thiolate Binding Sites", J. Biol. Inorg. Chem., 2006, 11, 876.

4. Magyar, J.S., Weng T.C., Stern, C.M., Dye, D., Rous, B.W., Payne, J.C., Bridgewater, B.M., Mijovilovich, A., Parkin, G., Zaleski, J.M., Penner-Hahn, J.E., Godwin, H.A., "Reexamination of Lead(II) coordination preferences in sulfer-rich sites: Implications for a critical mechanism of lead poisoning", J. Am. Chem. Soc., 2005, 127, 9495.

5. Masip, L., Pan, J., Haldar, S., Penner-Hahn, J.E., DeLisa, M.P., Georgiou, G., Bardwell, J. and Collet, J-F., "An Engineered Pathway for the Formation of Protein Disulfide Bonds", Science, 2004, 303, 1185.

6. Weng, T-C., Hiseh, W-Y., Uffelman, E.S., Gordon-Wylie, S.W., Collins, T.J., Pecoraro, V.L. and Penner-Hahn, J.E., "XANES Evidence Against a Manganyl Species in the S3 State of the Oxygen Evolving Complex", J. Am. Chem. Soc., 2004, 126, 8070.